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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD19 Recombinant antibody

CD19 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240135A3

Cat no : 83254-1-RR

Synonyms

AW495831, Cd19, CD19 antigen

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD19 Recombinant antibody (83254-1-RR)

    WB analysis using 83254-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83254-1-RR (CD19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity experiments of 83254-1-RR

    Affinity experiments of 83254-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83254-1-RR against Mouse CD19 were performed. The affinity constant is 0.303 nM.

Tested Applications

Positive WB detected inmouse spleen tissue, rat spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83254-1-RR targets CD19 in WB, ELISA applications and shows reactivity with Mouse, Rat samples.

Tested Reactivity Mouse, Rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD19 antigen
Calculated molecular weight60 kDa
Observed molecular weight62-70 kDa
GenBank accession numberNM_009844.2
Gene symbol Cd19
Gene ID (NCBI) 12478
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD19 is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

Protocols

Product Specific Protocols
WB protocol for CD19 antibody 83254-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols