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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Human CD19 (SJ25C1)

CD19 Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

SJ25C1

Cat no : CL647-65145

Synonyms

B lymphocyte antigen CD19, B4, CD19, CD19 molecule, Differentiation antigen CD19, T cell surface antigen Leu 12

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using CoraLite® Plus 647 Anti-Human CD19 (SJ25C1) (CL647-65145)

    FC experiment of human peripheral blood lymphocytes using CL647-65145

    100 ul human peripheral blood were surface stained with FITC Anti-Human CD3 (FITC-65151, Clone: UCHT1) and 5.00 ul CoraLite® Plus 647-conjugated Anti-Human CD19 (CL647-65145, Clone: SJ25C1). Lymphocytes were gated for analysis. Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using CoraLite® Plus 647 Anti-Human CD19 (SJ25C1) (CL647-65145)

    FC experiment of human peripheral blood lymphocytes using CL647-65145

    100 ul human peripheral blood were surface stained with FITC Anti-Human CD3 (FITC-65151, Clone: UCHT1) and CoraLite® Plus 647-conjugated Mouse IgG1 isotype control antibody. Lymphocytes were gated for analysis. Cells were not fixed.

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Tested Applications

Positive FC detected inhuman peripheral blood lymphocytes

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65145 targets CD19 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / Mouse IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen NALM1 + NALM16 cells 相同性解析による交差性が予測される生物種
Full Name CD19 molecule
Calculated molecular weight 556 aa, 61 kDa
GenBank accession numberBC006338
Gene symbol CD19
Gene ID (NCBI) 930
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CD19 antibody CL647-65145Download protocol
Standard Protocols
Click here to view our Standard Protocols