• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PE Anti-Human CD19 (SJ25C1)

CD19 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

SJ25C1

Cat no : PE-65145

Synonyms

B lymphocyte antigen CD19, B4, CD19, CD19 molecule, Differentiation antigen CD19, T cell surface antigen Leu 12

Validation Data Gallery

Filter:
  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using PE Anti-Human CD19 (SJ25C1) (PE-65145)

    FC experiment of human peripheral blood lymphocytes using PE-65145

    1X10^6 human peripheral blood lymphocytes were surface stained with 0.25 ug PE-anti-human CD19 (PE-65145, clone SJ25C1) (red) or 0.25 ug PE-mouse IgG1 isotype control (blue). Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using PE Anti-Human CD19 (SJ25C1) (PE-65145)

    FC experiment of human peripheral blood lymphocytes using PE-65145

    0.1 mL human whole blood cells were surface stained with 0.25 ug APC-anti-human CD3 (APC-65151, clone UCHT1) and 0.25 ug PE-anti-human CD19 (PE-65145, clone SJ25C1). Cells were then treated with red blood cell lysis buffer and were gated for lymphocytes for analysis of CD3 and CD19 staining. Cells were not fixed.

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive FC detected inHuman peripheral blood lymphocytes

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65145 targets CD19 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen NALM1 + NALM16 cells 相同性解析による交差性が予測される生物種
Full Name CD19 molecule
Calculated molecular weight 556 aa, 61 kDa
GenBank accession numberBC006338
Gene symbol CD19
Gene ID (NCBI) 930
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

Protocols

Product Specific Protocols
FC protocol for PE CD19 antibody PE-65145Download protocol
Standard Protocols
Click here to view our Standard Protocols