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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CD1d Polyclonal antibody (17336-1-AP)

    WB analysis of Jurkat cells using 17336-1-AP

    WB result of CD1D antibody (17336-1-AP, 1:500) with si-Control and si-CD1D transfected Jurkat cells.

  • Western Blot (WB) analysis of mouse skin tissue using CD1d Polyclonal antibody (17336-1-AP)

    WB analysis of mouse skin using 17336-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 17336-1-AP (CD1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

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  • KD/KO Validated

CD1d Polyclonal antibody

CD1d Polyclonal Antibody for WB, ELISA
Cat No. 17336-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, ELISA

Synonyms

CD1A, CD1D, CD1d molecule, R3, R3G1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse skin tissue, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17336-1-AP targets CD1d in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD1d fusion protein Ag11371 相同性解析による交差性が予測される生物種
Full Name CD1d molecule
Calculated molecular weight 335 aa, 38 kDa
Observed molecular weight 38-40 kDa, 50-55 kDa
GenBank accession numberBC027926
Gene Symbol CD1D
Gene ID (NCBI) 912
RRIDAB_2878387
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15813
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD1d is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. CD1d is an antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on NKT cells. When activated, NKT cells rapidly produce Th1 and Th2 cytokines. The molecular weight of unglycosylated CD1d is 38 kDa, while glycosylated form of CD1d is 48-50 kDa.

Protocols

Product Specific Protocols
WB protocol for CD1d antibody 17336-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB,IF

Emerg Microbes Infect

CD1d facilitates African swine fever virus entry into the host cells via clathrin-mediated endocytosis

Authors - Xin Chen
View Article