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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD2 Polyclonal antibody, PBS Only

CD2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10299-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD2 Polyclonal antibody (10299-1-AP)

    WB analysis using 10299-1-AP (same clone as 10299-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10299-1-AP (CD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10299-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD2 Polyclonal antibody (10299-1-AP)

    IHC staining of human tonsillitis using 10299-1-AP (same clone as 10299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10299-1-AP (CD2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 10299-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD2 Polyclonal antibody (10299-1-AP)

    IHC staining of human tonsillitis using 10299-1-AP (same clone as 10299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10299-1-AP (CD2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 10299-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10299-1-PBS targets CD2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD2 fusion protein Ag0423 相同性解析による交差性が予測される生物種
Full Name CD2 molecule
Calculated molecular weight 351 aa, 39 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC033583
Gene symbol CD2
Gene ID (NCBI) 914
RRIDAB_2228874
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.