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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD2 Polyclonal antibody

CD2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31803-1-AP

Synonyms

LFA-3 receptor, LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD2 Polyclonal antibody (31803-1-AP)

    WB analysis using 31803-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 31803-1-AP (CD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD2 Polyclonal antibody (31803-1-AP)

    IHC staining of human tonsillitis using 31803-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31803-1-AP (CD2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD2 Polyclonal antibody (31803-1-AP)

    IHC staining of human tonsillitis using 31803-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31803-1-AP (CD2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CD2 Polyclonal antibody (31803-1-AP)

    IF Staining of Jurkat using 31803-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD2 antibody (31803-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31803-1-AP targets CD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD2 molecule
Calculated molecular weight 351 aa, 39 kDa
Observed molecular weight45-50 kDa
GenBank accession numberBC033583
Gene symbol CD2
Gene ID (NCBI) 914
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

Protocols

Product Specific Protocols
WB protocol for CD2 antibody 31803-1-APDownload protocol
IHC protocol for CD2 antibody 31803-1-APDownload protocol
IF protocol for CD2 antibody 31803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols