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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CD2 Monoclonal antibody (60005-1-Ig)

    WB analysis of Jurkat using 60005-1-Ig (same clone as 60005-1-PBS)

    Untreated and PNGase F-treated lysates of Jurkat cells were subjected to SDS PAGE followed by western blot with 60005-1-Ig (CD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 60005-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CD2 Monoclonal antibody (60005-1-Ig)

    WB analysis of Jurkat using 60005-1-Ig (same clone as 60005-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60005-1-Ig (CD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60005-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human spleen tissue using CD2 Monoclonal antibody (60005-1-Ig)

    WB analysis of human spleen using 60005-1-Ig (same clone as 60005-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 60005-1-Ig (CD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60005-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD2 Monoclonal antibody (60005-1-Ig)

    IHC staining of human tonsillitis using 60005-1-Ig (same clone as 60005-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60005-1-Ig (CD2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60005-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD2 Monoclonal antibody (60005-1-Ig)

    IHC staining of human tonsillitis using 60005-1-Ig (same clone as 60005-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60005-1-Ig (CD2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60005-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51114-3

    Cytometric bead array standard curve of MP51114-3

    Cytometric bead array standard curve of MP51114-3, CD2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60005-1-PBS. Detection antibody: 60781-4-PBS. Standard:Eg0138. Range: 6.25-100 ng/mL.

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CD2 Monoclonal antibody, PBS Only (Capture)

CD2 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 60005-1-PBS
Clone No.3A10B2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor, CD2 molecule

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60005-1-PBS targets CD2 as part of a matched antibody pair:

MP51114-3: 60005-1-PBS capture and 60781-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD2 fusion protein Ag0423 相同性解析による交差性が予測される生物種
Full Name CD2 molecule
Calculated molecular weight 351 aa, 39 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC033583
Gene Symbol CD2
Gene ID (NCBI) 914
ENSEMBL Gene IDENSG00000116824
RRIDAB_2881350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP06729
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.