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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse CD2 Recombinant antibody, PBS Only (Detector)

CD2 Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241548D7

Cat no : 84360-4-PBS

Synonyms

T-cell surface antigen CD2, Lymphocyte antigen 37, Ly-37, LFA-3 receptor, LFA-2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD2 Recombinant antibody (84360-4-RR)

    WB analysis using 84360-4-RR (same clone as 84360-4-PBS)

    CTLL-2 cells were subjected to SDS PAGE followed by western blot with 84360-4-RR (CD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84360-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01249-1

    Sandwich ELISA standard curve of MP01249-1

    Sandwich ELISA standard curve of MP01249-1, Mouse CD2 Recombinant Matched Antibody Pair - PBS only. 84360-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1403. 84360-4-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL.

  • Sample test of MP01249-1

    Sample test of MP01249-1

    The mean CD2 concentration was determined to be 3.0 ng/mL in CTLL-2 cell extract based on a 0.9 mg/mL extract load.

  • Immunohistochemistry (IHC) staining of mouse thymus tissue using CD2 Recombinant antibody (84360-4-RR)

    IHC staining of mouse thymus using 84360-4-RR (same clone as 84360-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 84360-4-RR (CD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84360-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse thymus tissue using CD2 Recombinant antibody (84360-4-RR)

    IHC staining of mouse thymus using 84360-4-RR (same clone as 84360-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 84360-4-RR (CD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84360-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01249-1

    Cytometric bead array standard curve of MP01249-1

    Cytometric bead array standard curve of MP01249-1, MOUSE CD2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84360-5-PBS. Detection antibody: 84360-4-PBS. Standard: Eg1403. Range: 0.313-40 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84360-4-PBS targets CD2 as part of a matched antibody pair:

MP01249-1: 84360-5-PBS capture and 84360-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein 相同性解析による交差性が予測される生物種
Full Name CD2 antigen
Calculated molecular weight38 kDa
Observed molecular weight60 kDa
GenBank accession numberNM_013486.2
Gene symbol CD2
Gene ID (NCBI) 12481
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.