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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD20 Polyclonal antibody (31909-1-AP)

    WB analysis using 31909-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31909-1-AP (CD20 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD20 Polyclonal antibody (31909-1-AP)

    IHC staining of mouse spleen using 31909-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31909-1-AP (CD20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD20 Polyclonal antibody (31909-1-AP)

    IHC staining of mouse spleen using 31909-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31909-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat spleen tissue using CD20 Polyclonal antibody (31909-1-AP)

    IHC staining of rat spleen using 31909-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 31909-1-AP (CD20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat spleen tissue using CD20 Polyclonal antibody (31909-1-AP)

    IHC staining of rat spleen using 31909-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 31909-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using CD20 Polyclonal antibody (31909-1-AP)

    IF Staining of mouse colon using 31909-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using CD20 antibody (31909-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using CD20 Polyclonal antibody (31909-1-AP)

    IF Staining of mouse colon using 31909-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using CD20 antibody (31909-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CD20 Polyclonal antibody (31909-1-AP)

    IF Staining of mouse spleen using 31909-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD20 antibody (31909-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CD20 Polyclonal antibody (31909-1-AP)

    IF Staining of mouse spleen using 31909-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD20 antibody (31909-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat spleen tissue using CD20 Polyclonal antibody (31909-1-AP)

    IF Staining of rat spleen using 31909-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD20 antibody (31909-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat spleen tissue using CD20 Polyclonal antibody (31909-1-AP)

    IF Staining of rat spleen using 31909-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD20 antibody (31909-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CD20 Polyclonal antibody

CD20 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 31909-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Membrane-spanning 4-domains subfamily A member 1, Lymphocyte antigen 44, B-lymphocyte antigen CD20, B-cell differentiation antigen Ly-44

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse spleen tissue, rat spleen tissue
Positive IHC detected inmouse spleen tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue, mouse colon tissue, rat spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31909-1-AP targets CD20 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD20 fusion protein Ag36918 相同性解析による交差性が予測される生物種
Full Name membrane-spanning 4-domains, subfamily A, member 1
Calculated molecular weight32 kDa
Observed molecular weight35 kDa
GenBank accession numberNM_007641
Gene Symbol Ms4a1
Gene ID (NCBI) 12482
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP19437
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as a calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.

Protocols

Product Specific Protocols
WB protocol for CD20 antibody 31909-1-APDownload protocol
IHC protocol for CD20 antibody 31909-1-APDownload protocol
IF protocol for CD20 antibody 31909-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols