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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD20 Monoclonal antibody, PBS Only

CD20 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4A7G3

Cat no : 60271-1-PBS

Synonyms

Bp35, B-lymphocyte surface antigen B1, B-lymphocyte antigen CD20, B1, B lymphocyte antigen CD20

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD20 Monoclonal antibody (60271-1-Ig)

    WB analysis using 60271-1-Ig (same clone as 60271-1-PBS)

    Raji cells (CD20+), Daudi cells (CD20+), Ramos cells (CD20+), Jurkat cells (CD20-), and HeLa cells (CD20-) were subjected to SDS PAGE followed by western blot with 60271-1-Ig (CD20 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD20 Monoclonal antibody (60271-1-Ig)

    IHC staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60271-1-Ig (CD20 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD20 Monoclonal antibody (60271-1-Ig)

    IHC staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60271-1-Ig (CD20 antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD20 Monoclonal antibody (60271-1-Ig)

    IHC staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60271-1-Ig (CD20 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD20 Monoclonal antibody (60271-1-Ig)

    IHC staining of human appendicitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60271-1-Ig (CD20 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD20 Monoclonal antibody (60271-1-Ig)

    IHC staining of human appendicitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60271-1-Ig (CD20 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD20 Monoclonal antibody (60271-1-Ig)

    IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (60271-1-Ig, Clone: 4A7G3 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), CD3 antibody (17617-1-AP, green). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD20 Monoclonal antibody (60271-1-Ig)

    IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (60271-1-Ig, Clone: 4A7G3 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), CD3 antibody (17617-1-AP, green). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD20 Monoclonal antibody (60271-1-Ig)

    IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (60271-1-Ig, Clone: 4A7G3 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), CD3 antibody (17617-1-AP, green). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD20 Monoclonal antibody (60271-1-Ig)

    IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60271-1-Ig (CD20 antibody) at dilution of 1:500 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD20 Monoclonal antibody (60271-1-Ig)

    IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60271-1-Ig (CD20 antibody) at dilution of 1:500 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD20 Monoclonal antibody (60271-1-Ig)

    IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 18106-1-AP (PD-1/CD279 rabbit polyclonal antibody) at dilution of 1:100 and 60271-1-Ig (CD20 mouse monoclonal antibody) at dilution of 1:200, further stained with CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L) for 18106-1-AP, and CoraLite594-Conjugated Goat Anti-Mouse IgG(H+L) for 60271-1-Ig. This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD20 Monoclonal antibody (60271-1-Ig)

    IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 18106-1-AP (PD-1/CD279 rabbit polyclonal antibody) at dilution of 1:100 and 60271-1-Ig (CD20 mouse monoclonal antibody) at dilution of 1:200, further stained with CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L) for 18106-1-AP, and CoraLite594-Conjugated Goat Anti-Mouse IgG(H+L) for 60271-1-Ig. This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using CD20 Monoclonal antibody (60271-1-Ig)

    IF Staining of Raji using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD20 antibody (60271-1-Ig, Clone: 4A7G3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using CD20 Monoclonal antibody (60271-1-Ig)

    IF Staining of Raji using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD20 antibody (60271-1-Ig, Clone: 4A7G3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60271-1-PBS targets CD20 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD20 fusion protein Ag17309 相同性解析による交差性が予測される生物種
Full Name membrane-spanning 4-domains, subfamily A, member 1
Calculated molecular weight 297 aa, 33 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC002807
Gene symbol CD20
Gene ID (NCBI) 931
RRIDAB_2881391
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.