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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD200 Polyclonal antibody

CD200 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 31444-1-AP

Synonyms

OX2, Mox2, CD200 antigen

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CD200 Polyclonal antibody (31444-1-AP)

    WB analysis using 31444-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31444-1-AP (CD200 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD200 Polyclonal antibody (31444-1-AP)

    IHC staining of mouse spleen using 31444-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31444-1-AP (CD200 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD200 Polyclonal antibody (31444-1-AP)

    IHC staining of mouse spleen using 31444-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31444-1-AP (CD200 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CD200 Polyclonal antibody (31444-1-AP)

    IHC staining of mouse brain using 31444-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31444-1-AP (CD200 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CD200 Polyclonal antibody (31444-1-AP)

    IHC staining of mouse brain using 31444-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31444-1-AP (CD200 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, mouse lung tissue, mouse spleen tissue, mouse thymus tissue, rat brain tissue
Positive IHC detected inmouse spleen tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31444-1-AP targets CD200 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD200 antigen
Calculated molecular weight31 kDa
Observed molecular weight40-50 kDa
Gene symbol Cd200
Gene ID (NCBI) 17470
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).

Protocols

Product Specific Protocols
WB protocol for CD200 antibody 31444-1-APDownload protocol
IHC protocol for CD200 antibody 31444-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols