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  • Featured Product
  • KD/KO Validated

CD200 Monoclonal antibody, PBS Only

CD200 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5F3D6

Cat no : 66282-1-PBS

Synonyms

CD200, CD200 molecule, MOX1, MOX2, MRC, OX 2, OX 2 membrane glycoprotein

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using CD200 Monoclonal antibody (66282-1-Ig)

    WB analysis of fetal human brain using 66282-1-Ig (same clone as 66282-1-PBS)

    Untreated and PNGase F-treated lysates of fetal human brain tissue and human spleen tissue were subjected to SDS PAGE followed by western blot with 66282-1-Ig (CD200 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using CD200 Monoclonal antibody (66282-1-Ig)

    WB analysis of fetal human brain using 66282-1-Ig (same clone as 66282-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66282-1-Ig (CD200 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human spleen tissue using CD200 Monoclonal antibody (66282-1-Ig)

    WB analysis of human spleen using 66282-1-Ig (same clone as 66282-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66282-1-Ig (CD200 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD200 Monoclonal antibody (66282-1-Ig)

    IHC staining of human tonsillitis using 66282-1-Ig (same clone as 66282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66282-1-Ig (CD200 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD200 Monoclonal antibody (66282-1-Ig)

    IHC staining of human tonsillitis using 66282-1-Ig (same clone as 66282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66282-1-Ig (CD200 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66282-1-PBS targets CD200 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD200 fusion protein Ag5045 相同性解析による交差性が予測される生物種
Full Name CD200 molecule
Calculated molecular weight 269 aa, 30 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC022522
Gene symbol CD200
Gene ID (NCBI) 4345
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).