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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD200 Recombinant antibody

CD200 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240391A8

Cat no : 83437-5-RR

Synonyms

OX2, Mox2, CD200 antigen, 240391A8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD200 Recombinant antibody (83437-5-RR)

    WB analysis using 83437-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83437-5-RR (CD200 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CD200 Recombinant antibody (83437-5-RR)

    IHC staining of mouse brain using 83437-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83437-5-RR (CD200 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CD200 Recombinant antibody (83437-5-RR)

    IHC staining of mouse brain using 83437-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83437-5-RR (CD200 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using CD200 Recombinant antibody (83437-5-RR)

    IHC staining of mouse cerebellum using 83437-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83437-5-RR (CD200 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using CD200 Recombinant antibody (83437-5-RR)

    IHC staining of mouse cerebellum using 83437-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83437-5-RR (CD200 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83437-5-RR

    Affinity and Kinetic Characterization of 83437-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83437-5-RR against Mouse CD200 were performed. The affinity constant is 28.8 nM.

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Tested Applications

Positive WB detected inmouse cerebellum tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83437-5-RR is CD200 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD200 antigen
Calculated molecular weight31 kDa
Observed molecular weight40 kDa
Gene symbol Cd200
Gene ID (NCBI) 17470
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).

Protocols

Product Specific Protocols
WB protocol for CD200 antibody 83437-5-RRDownload protocol
IHC protocol for CD200 antibody 83437-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols