• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD200 Recombinant antibody

CD200 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240391A8

Cat no : 83437-5-RR

Synonyms

OX2, Mox2, CD200 antigen, 240391A8

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CD200 Recombinant antibody (83437-5-RR)

    WB analysis using 83437-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83437-5-RR (CD200 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CD200 Recombinant antibody (83437-5-RR)

    IHC staining of mouse brain using 83437-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83437-5-RR (CD200 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CD200 Recombinant antibody (83437-5-RR)

    IHC staining of mouse brain using 83437-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83437-5-RR (CD200 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using CD200 Recombinant antibody (83437-5-RR)

    IHC staining of mouse cerebellum using 83437-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83437-5-RR (CD200 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using CD200 Recombinant antibody (83437-5-RR)

    IHC staining of mouse cerebellum using 83437-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83437-5-RR (CD200 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83437-5-RR

    Affinity and Kinetic Characterization of 83437-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83437-5-RR against Mouse CD200 were performed. The affinity constant is 28.8 nM.

☆ 3/31（月）まで！二次抗体 & FlexAbleが30%オフ！ >> 免疫アッセイサポートキャンペーン

Tested Applications

Positive WB detected inmouse cerebellum tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83437-5-RR is CD200 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD200 antigen
Calculated molecular weight31 kDa
Observed molecular weight40 kDa
Gene symbol Cd200
Gene ID (NCBI) 17470
RRIDAB_3671078
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).

Protocols

Product Specific Protocols
WB protocol for CD200 antibody 83437-5-RRDownload protocol
IHC protocol for CD200 antibody 83437-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols