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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD200 Rabbit Recombinant Antibody

CD200 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241347E1

Cat no : 98091-1-RR

Synonyms

OX 2, MRC, MOX2, MOX1, CD200 molecule

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD200 Rabbit Recombinant Antibody (98091-1-RR)

    FC experiment of human PBMCs using 98091-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD200 Rabbit Recombinant Antibody (98091-1-RR, Clone:241347E1) or 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with CoraLite® Plus 647 Anti-Human CD19 (4G7). Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD200 Rabbit Recombinant Antibody (98091-1-RR)

    FC experiment of human PBMCs using 98091-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD200 Rabbit Recombinant Antibody (98091-1-RR, Clone:241347E1) (red) or 0.25 ug Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD200 Rabbit Recombinant Antibody (98091-1-RR)

    FC experiment of human PBMCs using 98091-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD200 Rabbit Recombinant Antibody (98091-1-RR, Clone:241347E1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), and 0.25 ug Isotype Control, and 0.25 ug CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98091-1-RR

    Affinity and Kinetic Characterization of 98091-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98091-1-RR against Human CD200 were performed. The affinity constant is 1.15 nM.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98091-1-RR targets CD200 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name CD200 molecule
Calculated molecular weight 269 aa, 30 kDa
GenBank accession numberBC022522
Gene symbol CD200
Gene ID (NCBI) 4345
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).

Protocols

Product Specific Protocols
FC protocol for CD200 antibody 98091-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols