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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD206 Recombinant antibody, PBS Only

CD206 Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G15

Cat no : 81525-1-PBS

Synonyms

hMR, C-type lectin domain family 13 member D-like, C-type lectin domain family 13 member D, CLEC13DL, CLEC13D

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD206 Recombinant antibody (81525-1-RR)

    WB analysis using 81525-1-RR (same clone as 81525-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81525-1-RR (CD206 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD206 Recombinant antibody (81525-1-RR)

    IHC staining of human lung cancer using 81525-1-RR (same clone as 81525-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD206 Recombinant antibody (81525-1-RR)

    IHC staining of human lung cancer using 81525-1-RR (same clone as 81525-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD206 Recombinant antibody (81525-1-RR)

    IHC staining of human colon cancer using 81525-1-RR (same clone as 81525-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD206 Recombinant antibody (81525-1-RR)

    IHC staining of human colon cancer using 81525-1-RR (same clone as 81525-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD206 Recombinant antibody (81525-1-RR)

    IHC staining of human lymphoma using 81525-1-RR (same clone as 81525-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD206 Recombinant antibody (81525-1-RR)

    IHC staining of human lung cancer using 81525-1-RR (same clone as 81525-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD206 Recombinant antibody (81525-1-RR)

    IHC staining of human lung cancer using 81525-1-RR (same clone as 81525-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD206 Recombinant antibody (81525-1-RR)

    IHC staining of human placenta using 81525-1-RR (same clone as 81525-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD206 Recombinant antibody (81525-1-RR)

    IHC staining of human placenta using 81525-1-RR (same clone as 81525-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD206 Recombinant antibody (81525-1-RR)

    IHC staining of human lymphoma using 81525-1-RR (same clone as 81525-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using CD206 Recombinant antibody (81525-1-RR)

    IF Staining of human placenta using 81525-1-RR (same clone as 81525-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD206 antibody (81525-1-RR, Clone: 1G15 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using CD206 Recombinant antibody (81525-1-RR)

    IF Staining of human placenta using 81525-1-RR (same clone as 81525-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD206 antibody (81525-1-RR, Clone: 1G15 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse lung tissue using CD206 Recombinant antibody (81525-1-RR)

    IF Staining of mouse lung using 81525-1-RR (same clone as 81525-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using CD206 antibody (81525-1-RR, Clone: 1G15 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse lung tissue using CD206 Recombinant antibody (81525-1-RR)

    IF Staining of mouse lung using 81525-1-RR (same clone as 81525-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using CD206 antibody (81525-1-RR, Clone: 1G15 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81525-1-PBS targets CD206 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mannose receptor, C type 1
Calculated molecular weight 166 kDa
Observed molecular weight170 kDa
GenBank accession numberNM_002438
Gene symbol CD206
Gene ID (NCBI) 4360
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia.