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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD209 Polyclonal antibody (31621-1-AP)

    WB analysis using 31621-1-AP

    Human placenta tissue was subjected to SDS PAGE followed by western blot with 31621-1-AP (CD209 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD209 Polyclonal antibody (31621-1-AP)

    IHC staining of human tonsillitis using 31621-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31621-1-AP (CD209 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD209 Polyclonal antibody (31621-1-AP)

    IHC staining of human tonsillitis using 31621-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31621-1-AP (CD209 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CD209 Polyclonal antibody

CD209 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31621-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

DC-SIGN1, DC SIGN1, DC SIGN, C-type lectin domain family 4 member L, CLEC4L

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31621-1-AP targets CD209 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD209 molecule
Calculated molecular weight 404 aa, 45.7 kDa
Observed molecular weight45 kDa
GenBank accession numberBC110615
Gene Symbol CD209
Gene ID (NCBI) 30835
RRIDAB_3670051
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9NNX6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD209 (DC-SIGN, CLEC4L) is a C-type lectin receptor that is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens, including bacteria, viruses, and parasites. CD209 is preferentially expressed on dendritic cells (DCs). It mediates transient adhesion of DCs with T cells. It has been reported that CD209 plays a role in capture and transmission of HIV from DC to T cells (PMID: 10721995).

Protocols

Product Specific Protocols
WB protocol for CD209 antibody 31621-1-APDownload protocol
IHC protocol for CD209 antibody 31621-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols