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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD21 Polyclonal antibody

CD21 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 28206-1-AP

Synonyms

C3DR, CD21, Complement C3d receptor, Complement receptor type 2, CR2, EBV receptor, Epstein Barr virus receptor, SLEB9

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD21 Polyclonal antibody (28206-1-AP)

    IHC staining of human tonsillitis using 28206-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28206-1-AP (CD21 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD21 Polyclonal antibody (28206-1-AP)

    IHC staining of human tonsillitis using 28206-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28206-1-AP (CD21 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD21 Polyclonal antibody (28206-1-AP)

    IF Staining of human tonsillitis using 28206-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD21 antibody (28206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD21 Polyclonal antibody (28206-1-AP)

    IF Staining of human tonsillitis using 28206-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD21 antibody (28206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28206-1-AP targets CD21 in IHC, IF-P, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD21 fusion protein Ag27971 相同性解析による交差性が予測される生物種
Full Name complement component (3d/Epstein Barr virus) receptor 2
Calculated molecular weight 1092 aa, 119 kDa
GenBank accession numberBC136394
Gene symbol CD21
Gene ID (NCBI) 1380
RRIDAB_2881087
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.

Protocols

Product Specific Protocols
IHC protocol for CD21 antibody 28206-1-APDownload protocol
IF protocol for CD21 antibody 28206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols