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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD21 (BU32) Mouse IgG1 Recombinant Antibody, PBS Only

CD21 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

BU32

Cat no : 65512-1-PBS

Synonyms

C3DR, CD21, Complement C3d receptor, Complement receptor type 2, CR2, EBV receptor, Epstein Barr virus receptor, SLEB9

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD21 (BU32) Mouse IgG1 Recombinant Ant (65512-1-PBS)

    FC experiment of human PBMCs using 65512-1-PBS

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD21 Mouse Recombinant Antibody (65512-1-PBS, Clone:BU32) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-Conjugated Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD21 (BU32) Mouse IgG1 Recombinant Ant (65512-1-PBS)

    FC experiment of human PBMCs using 65512-1-PBS

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD21 Mouse Recombinant Antibody (65512-1-PBS, Clone:BU32) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD21 (BU32) Mouse IgG1 Recombinant Ant (65512-1-PBS)

    FC experiment of human PBMCs using 65512-1-PBS

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD21 Mouse Recombinant Antibody (65512-1-PBS, Clone:BU32) (left) or 0.1 ug Anti-Human CD21 Mouse Monoclonal Antibody (Clone:BU32) (center and right) and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-Conjugated Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65512-1-PBS targets CD21 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Recombinant
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name complement component (3d/Epstein Barr virus) receptor 2
Calculated molecular weight 1092 aa, 119 kDa
GenBank accession numberBC136394
Gene symbol CD21
Gene ID (NCBI) 1380
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C. Stable for one year after shipment.

Background Information

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.

Protocols

Product Specific Protocols
FC protocol for CD21 antibody 65512-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols