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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD21 (BU32) Mouse IgG2a Recombinant Antibody

CD21 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

BU32

Cat no : 65591-1-MR

Synonyms

C3DR, CD21, Complement C3d receptor, Complement receptor type 2, CR2, EBV receptor, Epstein Barr virus receptor, SLEB9

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD21 (BU32) Mouse IgG2a Recombinant Ant (65591-1-MR)

    FC experiment of human PBMCs using 65591-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD21 Mouse IgG2a Recombinant Antibody (65591-1-MR, Clone: BU32) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD21 (BU32) Mouse IgG2a Recombinant Ant (65591-1-MR)

    FC experiment of human PBMCs using 65591-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD21 Mouse IgG2a Recombinant Antibody (65591-1-MR, Clone: BU32) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD21 (BU32) Mouse IgG2a Recombinant Ant (65591-1-MR)

    FC experiment of human PBMCs using 65591-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD21 Mouse IgG2a Recombinant Antibody (65591-1-MR, Clone: BU32) (left) or 0.25 ug Anti-Human CD21 (65198-1-Ig, Clone: BU32) (right) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65591-1-MR targets CD21 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name complement component (3d/Epstein Barr virus) receptor 2
Calculated molecular weight 1092 aa, 119 kDa
GenBank accession numberBC136394
Gene symbol CD21
Gene ID (NCBI) 1380
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.

Protocols

Product Specific Protocols
FC protocol for CD21 antibody 65591-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols