CD22 Monoclonal antibody, PBS Only (Capture)
CD22 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
7A2F1
Cat no : 66103-1-PBS
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
Application | Dilution |
---|---|
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
Product Information
66103-1-PBS targets CD22 as part of a matched antibody pair:
MP50311-4: 66103-1-PBS capture and 66103-6-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | CD22 fusion protein Ag17986 相同性解析による交差性が予測される生物種 |
Full Name | CD22 molecule |
Calculated molecular weight | 847 aa, 95 kDa |
Observed molecular weight | 135 kDa |
GenBank accession number | BC109306 |
Gene symbol | CD22 |
Gene ID (NCBI) | 933 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
Background Information
CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.