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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD22 Recombinant antibody (84265-5-RR)

    WB analysis using 84265-5-RR (same clone as 84265-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84265-5-RR (CD22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84265-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD22 Recombinant antibody (84265-5-RR)

    IHC staining of human tonsillitis using 84265-5-RR (same clone as 84265-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84265-5-RR (CD22 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84265-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD22 Recombinant antibody (84265-5-RR)

    IHC staining of human tonsillitis using 84265-5-RR (same clone as 84265-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84265-5-RR (CD22 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84265-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84265-5-RR

    Affinity and Kinetic Characterization of 84265-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84265-5-RR against Human CD22 were performed. The affinity constant is 262 nM.

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CD22 Recombinant antibody, PBS Only

CD22 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84265-5-PBS
Clone No.241314B5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

CD22 molecule, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22, B cell receptor CD22

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84265-5-PBS targets CD22 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name CD22 molecule
Calculated molecular weight 847 aa, 95 kDa
Observed molecular weight140 kDa
GenBank accession numberBC109306
Gene Symbol CD22
Gene ID (NCBI) 933
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP20273
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.