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Validation Data Gallery

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  • Western Blot (WB) analysis of human peripheral blood platelets using CD226 Recombinant antibody (82991-3-RR)

    WB analysis of human peripheral blood platelets using 82991-3-RR (same clone as 82991-3-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 82991-3-RR (CD226 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82991-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD226 Recombinant antibody (82991-3-RR)

    IHC staining of human tonsillitis using 82991-3-RR (same clone as 82991-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82991-3-RR (CD226 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82991-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEL cells using CD226 Recombinant antibody (82991-3-RR)

    IF Staining of HEL using 82991-3-RR (same clone as 82991-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEL cells using CD226 antibody (82991-3-RR, Clone: 230041E12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82991-3-PBS in a different storage buffer formulation.

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CD226 Recombinant antibody, PBS Only

CD226 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82991-3-PBS
Clone No.230041E12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DNAM-1, DNAM1, DNAM 1, CD226 molecule, CD226 antigen

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82991-3-PBS targets CD226 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD226 molecule
Calculated molecular weight 336 aa, 39 kDa
Observed molecular weight65 kDa
GenBank accession numberBC074787
Gene Symbol CD226
Gene ID (NCBI) 10666
ENSEMBL Gene IDENSG00000150637
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ15762
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD226 (DNAM-1) is a ~65 kDa glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. CD226 mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Interactions of CD226 and its ligands, CD155 and CD112, induce NK and T cell-mediated cytotoxicity and cytokine secretion (PMID: 15039383).