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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD226 Recombinant antibody

CD226 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240673G4

Cat no : 82991-4-RR

Synonyms

DNAM-1, DNAM1, DNAM 1, CD226 molecule, CD226 antigen

Validation Data Gallery

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  • Western Blot (WB) analysis of human peripheral blood platelets using CD226 Recombinant antibody (82991-4-RR)

    WB analysis of human peripheral blood platelets using 82991-4-RR

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 82991-4-RR (CD226 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82991-4-RR

    Affinity and Kinetic Characterization of 82991-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82991-4-RR against Human CD226 were performed. The affinity constant is 2.42 nM.

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Tested Applications

Positive WB detected inhuman peripheral blood platelets

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82991-4-RR is CD226 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD226 molecule
Calculated molecular weight 336 aa, 39 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC074787
Gene symbol CD226
Gene ID (NCBI) 10666
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD226 (DNAM-1) is a ~65 kDa glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. CD226 mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Interactions of CD226 and its ligands, CD155 and CD112, induce NK and T cell-mediated cytotoxicity and cytokine secretion (PMID: 15039383).

Protocols

Product Specific Protocols
WB protocol for CD226 antibody 82991-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols