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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD226 Rabbit Recombinant Antibody, PBS Only

CD226 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240673G4

Cat no : 98002-2-PBS

Synonyms

DNAM-1, DNAM1, DNAM 1, CD226 molecule, CD226 antigen

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD226 Rabbit Recombinant Antibody (98002-2-RR)

    FC experiment of human PBMCs using 98002-2-RR (same clone as 98002-2-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD226 Rabbit Recombinant Antibody (98002-2-RR, Clone: 240673G4) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (CL647-65569, Clone: OKT3). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98002-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD226 Rabbit Recombinant Antibody (98002-2-RR)

    FC experiment of human PBMCs using 98002-2-RR (same clone as 98002-2-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD226 Rabbit Recombinant Antibody (98002-2-RR, Clone:240673G4) (red) or Isotype Control (blue) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98002-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98002-2-RR

    Affinity and Kinetic Characterization of 98002-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98036-1-RR against Human CD226 were performed. The affinity constant is 3.70 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 98002-2-PBS is CD226 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD226 molecule
Calculated molecular weight 336 aa, 39 kDa
GenBank accession numberBC074787
Gene symbol CD226
Gene ID (NCBI) 10666
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD226 (DNAM-1) is a ~65 kDa glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. CD226 mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Interactions of CD226 and its ligands, CD155 and CD112, induce NK and T cell-mediated cytotoxicity and cytokine secretion (PMID: 15039383).