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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD23 Polyclonal antibody

CD23 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 10300-1-AP

Synonyms

BLAST 2, CD23, CD23A, CLEC4J, Fc epsilon RII, FCE2, FCER2, IGEBF, Lymphocyte IgE receptor

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD23 Polyclonal antibody (10300-1-AP)

    IHC staining of human tonsillitis using 10300-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10300-1-AP (CD23 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD23 Polyclonal antibody (10300-1-AP)

    IHC staining of human tonsillitis using 10300-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10300-1-AP (CD23 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD23 Polyclonal antibody (10300-1-AP)

    IHC staining of human lymphoma using 10300-1-AP

    Immunohistochemical analysis of paraffin-embedded human diffuse small B-cell lymphoma using 10300-1-AP (CD23, FCER2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD23 Polyclonal antibody (10300-1-AP)

    IHC staining of human lymphoma using 10300-1-AP

    Immunohistochemical analysis of paraffin-embedded human diffuse small B-cell lymphoma using 10300-1-AP (CD23, FCER2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 10300-1-AP is CD23 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD23 fusion protein Ag0425 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgE, low affinity II, receptor for (CD23)
Calculated molecular weight 321 aa, 36 kDa
GenBank accession numberBC064417
Gene symbol CD23
Gene ID (NCBI) 2208
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.