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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD23 Monoclonal antibody

CD23 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

4C7C9

Cat no : 60208-2-Ig

Synonyms

BLAST 2, CD23, CD23A, CLEC4J, Fc epsilon RII, FCE2, FCER2, IGEBF, Lymphocyte IgE receptor

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using CD23 Monoclonal antibody (60208-2-Ig)

    WB analysis of Raji using 60208-2-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 60208-2-Ig (CD23 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD23 Monoclonal antibody (60208-2-Ig)

    IHC staining of human tonsillitis using 60208-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-2-Ig (CD23 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD23 Monoclonal antibody (60208-2-Ig)

    IHC staining of human tonsillitis using 60208-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-2-Ig (CD23 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD23 Monoclonal antibody (60208-2-Ig)

    IHC staining of human lymphoma using 60208-2-Ig

    Immunohistochemical analysis of paraffin-embedded human diffuse small B-cell lymphoma using 60208-2-Ig(CD23 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD23 Monoclonal antibody (60208-2-Ig)

    IHC staining of human lymphoma using 60208-2-Ig

    Immunohistochemical analysis of paraffin-embedded human diffuse small B-cell lymphoma using 60208-2-Ig(CD23 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD23 Monoclonal antibody (60208-2-Ig)

    IF Staining of human tonsillitis using 60208-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60208-2-Ig (CD23 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD23 Monoclonal antibody (60208-2-Ig)

    IF Staining of human tonsillitis using 60208-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60208-2-Ig (CD23 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inRaji cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60208-2-Ig targets CD23 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD23 fusion protein Ag0425 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgE, low affinity II, receptor for (CD23)
Calculated molecular weight 321 aa, 36 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC064417
Gene symbol CD23
Gene ID (NCBI) 2208
RRIDAB_11232221
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.

Protocols

Product Specific Protocols
WB protocol for CD23 antibody 60208-2-IgDownload protocol
IHC protocol for CD23 antibody 60208-2-IgDownload protocol
IF protocol for CD23 antibody 60208-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols