CD23 Monoclonal antibody
CD23 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
4C7C9
Cat no : 60208-2-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Raji cells |
Positive IHC detected in | human tonsillitis tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
60208-2-Ig targets CD23 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | CD23 fusion protein Ag0425 相同性解析による交差性が予測される生物種 |
Full Name | Fc fragment of IgE, low affinity II, receptor for (CD23) |
Calculated molecular weight | 321 aa, 36 kDa |
Observed molecular weight | 40 kDa |
GenBank accession number | BC064417 |
Gene symbol | CD23 |
Gene ID (NCBI) | 2208 |
RRID | AB_11232221 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.
Protocols
Product Specific Protocols | |
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WB protocol for CD23 antibody 60208-2-Ig | Download protocol |
IHC protocol for CD23 antibody 60208-2-Ig | Download protocol |
IF protocol for CD23 antibody 60208-2-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |