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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD23 Polyclonal antibody

CD23 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18642-1-AP

Synonyms

Fc epsilon RII, C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using CD23 Polyclonal antibody (18642-1-AP)

    WB analysis of Raji using 18642-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 18642-1-AP (FCER2,CD23 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD23 Polyclonal antibody (18642-1-AP)

    IHC staining of human tonsillitis using 18642-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18642-1-AP (CD23 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD23 Polyclonal antibody (18642-1-AP)

    IHC staining of human tonsillitis using 18642-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18642-1-AP (CD23 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD23 Polyclonal antibody (18642-1-AP)

    IHC staining of human lymphoma using 18642-1-AP

    Immunohistochemical analysis of paraffin-embedded human diffuse small B-cell lymphoma tissue slide using 18642-1-AP (FCER2,CD23 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD23 Polyclonal antibody (18642-1-AP)

    IHC staining of human lymphoma using 18642-1-AP

    Immunohistochemical analysis of paraffin-embedded human diffuse small B-cell lymphoma tissue slide using 18642-1-AP (FCER2,CD23 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD23 Polyclonal antibody (18642-1-AP)

    IHC staining of human tonsillitis using 18642-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18642-1-AP (FCER2,CD23 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD23 Polyclonal antibody (18642-1-AP)

    IHC staining of human tonsillitis using 18642-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18642-1-AP (FCER2,CD23 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

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Tested Applications

Positive WB detected inRaji cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18642-1-AP targets CD23 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD23 fusion protein Ag13348 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgE, low affinity II, receptor for (CD23)
Calculated molecular weight 36 kDa
Observed molecular weight 38-42 kDa
GenBank accession numberBC014108
Gene symbol CD23
Gene ID (NCBI) 2208
RRIDAB_2878555
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.

Protocols

Product Specific Protocols
WB protocol for CD23 antibody 18642-1-APDownload protocol
IHC protocol for CD23 antibody 18642-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols