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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD23 Rabbit Recombinant Antibody, PBS Only

CD23 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241270C6

Cat no : 98088-1-PBS

Synonyms

C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2, BLAST 2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD23 Rabbit Recombinant Antibody (98088-1-RR)

    FC experiment of human PBMCs using 98088-1-RR (same clone as 98088-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD23 Rabbit Recombinant Antibody (98088-1-RR, Clone:241270C6) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with CoraLite® Plus 647 Anti-Human CD19 (4G7). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98088-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD23 Rabbit Recombinant Antibody (98088-1-RR)

    FC experiment of human PBMCs using 98088-1-RR (same clone as 98088-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD23 Rabbit Recombinant Antibody (98088-1-RR, Clone:241270C6) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98088-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD23 Rabbit Recombinant Antibody (98088-1-RR)

    FC experiment of human PBMCs using 98088-1-RR (same clone as 98088-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD23 Rabbit Recombinant Antibody (98088-1-RR, Clone:241270C6) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), and 0.25 ug Isotype Control, and 0.25 ug CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). Cells were not fixed. This data was developed using the same antibody clone with 98088-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98088-1-RR

    Affinity and Kinetic Characterization of 98088-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98088-1-RR against Human CD23 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98088-1-PBS targets CD23 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgE, low affinity II, receptor for (CD23)
Calculated molecular weight 321 aa, 36 kDa
GenBank accession numberBC064417
Gene symbol CD23
Gene ID (NCBI) 2208
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.