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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DARC Polyclonal antibody

DARC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55185-1-AP

Synonyms

CCBP1, CD234, DARC, Dfy, FY, Fy glycoprotein, Glycoprotein D, GPD, GpFy, Plasmodium vivax receptor, WBCQ1

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using DARC Polyclonal antibody (55185-1-AP)

    WB analysis of K-562 using 55185-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 55185-1-AP (DARC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using DARC Polyclonal antibody (55185-1-AP)

    IHC staining of human kidney using 55185-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55185-1-AP (DARC antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using DARC Polyclonal antibody (55185-1-AP)

    IHC staining of human kidney using 55185-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55185-1-AP (DARC antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inK-562 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55185-1-AP targets DARC in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name Duffy blood group, chemokine receptor
Calculated molecular weight 36 kDa
Observed molecular weight 33-35 kDa
GenBank accession numberNM_002036
Gene symbol DARC
Gene ID (NCBI) 2532
RRIDAB_10838809
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

CD234, also named as DARC, Duffy, FY and GPD, belongs to the G-protein coupled receptor Duffy family. It is a non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi. This antibody is specific to CD234.

Protocols

Product Specific Protocols
WB protocol for DARC antibody 55185-1-APDownload protocol
IHC protocol for DARC antibody 55185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

Ocul Surf

Single-cell RNA sequencing reveals the complex cellular niche of pterygium

Authors - Xueling Zhang
View Article