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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD24 Polyclonal antibody (10600-1-AP)

    WB analysis using 10600-1-AP (same clone as 10600-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10600-1-AP (CD24 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10600-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using CD24 Polyclonal antibody (10600-1-AP)

    WB analysis of mouse brain using 10600-1-AP (same clone as 10600-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10600-1-AP (CD24 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10600-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat spleen tissue using CD24 Polyclonal antibody (10600-1-AP)

    WB analysis of rat spleen using 10600-1-AP (same clone as 10600-1-PBS)

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 10600-1-AP (CD24 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10600-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD24 Polyclonal antibody (10600-1-AP)

    IHC staining of human appendicitis using 10600-1-AP (same clone as 10600-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10600-1-AP (CD24 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10600-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD24 Polyclonal antibody (10600-1-AP)

    IHC staining of human appendicitis using 10600-1-AP (same clone as 10600-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10600-1-AP (CD24 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10600-1-PBS in a different storage buffer formulation.

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CD24 Polyclonal antibody, PBS Only

CD24 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 10600-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

CD24A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10600-1-PBS targets CD24 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD24 fusion protein Ag0654 相同性解析による交差性が予測される生物種
Full Name CD24 molecule
Calculated molecular weight8 kDa
Observed molecular weight 30-70 kDa
GenBank accession numberBC007674
Gene Symbol CD24
Gene ID (NCBI) 100133941
RRIDAB_10646440
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP25063
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion. Since it is highly glycosylated, the apparent molecular weight of CD24 could be variable, ranging from 30 kDa to 70 kDa. (Ref: Akihiko Sano, MD., 2009)