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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD24 Monoclonal antibody, PBS Only

CD24 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H5C4

Cat no : 67627-1-PBS

Synonyms

CD24A, 1H5C4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD24 Monoclonal antibody (67627-1-Ig)

    WB analysis using 67627-1-Ig (same clone as 67627-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67627-1-Ig (CD24 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67627-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Daudi cells using CD24 Monoclonal antibody (67627-1-Ig)

    IF Staining of Daudi using 67627-1-Ig (same clone as 67627-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Daudi cells using CD24 antibody (67627-1-Ig, Clone: 1H5C4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67627-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67627-1-PBS targets CD24 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD24 fusion protein Ag11679 相同性解析による交差性が予測される生物種
Full Name CD24 molecule
Calculated molecular weight8 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC007674
Gene symbol CD24
Gene ID (NCBI) 100133941
RRIDAB_2882828
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion. Since it is highly glycosylated, the apparent molecular weight of CD24 could be variable, ranging from 30 kDa to 70 kDa. (Ref: Akihiko Sano, MD., 2009)