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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD244 Rabbit Recombinant Antibody, PBS Only

CD244 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241260G10

Cat no : 98133-1-PBS

Synonyms

SLAMF4, NKR2B4, NK cell activation-inducing ligand, NAIL, h2B4

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD244 Rabbit Recombinant Antibody (98133-1-RR)

    FC experiment of human PBMCs using 98133-1-RR (same clone as 98133-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD244 Rabbit Recombinant Antibody (98133-1-RR, Clone: 241260G10) or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98133-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD244 Rabbit Recombinant Antibody (98133-1-RR)

    FC experiment of human PBMCs using 98133-1-RR (same clone as 98133-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD244 Rabbit Recombinant Antibody (98133-1-RR, Clone:241260G10) (red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98133-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98133-1-RR

    Affinity and Kinetic Characterization of 98133-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98133-1-RR against Human CD244 were performed. The affinity constant is 1.29 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 98133-1-PBS is CD244 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD244 molecule, natural killer cell receptor 2B4
Calculated molecular weight 365 aa, 41 kDa
GenBank accession numberBC053985
Gene symbol CD244
Gene ID (NCBI) 51744
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD244, also known as SLAMF4 or 2B4, is a type I transmembrane glycoprotein belonging to the signaling lymphocyte activation molecule (SLAM) family. It consists of an extracellular segment with two immunoglobulin (Ig)-like domains, a transmembrane region, and a cytoplasmic domain containing tyrosine-based motifs (PMID: 9841922; 30546369). CD244 is present on natural killer (NK) cells, γδ T cells, a subset of CD8+ T cells, monocytes, basophils, dendritic cells, and myeloid-derived suppressor cells (MDSCs) (PMID: 30546369). It binds to CD48 with high affinity and transmits stimulatory or inhibitory signals that regulate immune function (PMID: 9841922; 18523281).