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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD247 Polyclonal antibody, PBS Only

CD247 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12837-2-PBS

Synonyms

T cell receptor T3 zeta chain, CD3Z, CD3Q, CD3H, CD3 ZETA

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CD247 Polyclonal antibody (12837-2-AP)

    WB analysis of Jurkat using 12837-2-AP (same clone as 12837-2-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12837-2-AP (CD247 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12837-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse spleen tissue using CD247 Polyclonal antibody (12837-2-AP)

    WB analysis of mouse spleen using 12837-2-AP (same clone as 12837-2-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12837-2-AP (CD247 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12837-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human spleen tissue using CD247 Polyclonal antibody (12837-2-AP)

    WB analysis of human spleen using 12837-2-AP (same clone as 12837-2-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 12837-2-AP (CD247 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12837-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using CD247 Polyclonal antibody (12837-2-AP)

    IP experiment of Jurkat using 12837-2-AP (same clone as 12837-2-PBS)

    IP result of anti-CD247 (IP:12837-2-AP, 3ug; Detection:12837-2-AP 1:700) with Jurkat cells lysate 1200ug. This data was developed using the same antibody clone with 12837-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD247 Polyclonal antibody (12837-2-AP)

    IHC staining of human tonsillitis using 12837-2-AP (same clone as 12837-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12837-2-AP (CD247 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12837-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD247 Polyclonal antibody (12837-2-AP)

    IHC staining of human tonsillitis using 12837-2-AP (same clone as 12837-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12837-2-AP (CD247 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12837-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD247 Polyclonal antibody (12837-2-AP)

    IHC staining of human tonsillitis using 12837-2-AP (same clone as 12837-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12837-2-AP (CD247 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12837-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CD247 Polyclonal antibody (12837-2-AP)

    IF Staining of Jurkat using 12837-2-AP (same clone as 12837-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using 12837-2-AP (CD247 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12837-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12837-2-PBS targets CD247 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD247 fusion protein Ag3889 相同性解析による交差性が予測される生物種
Full Name CD247 molecule
Calculated molecular weight 16 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC025703
Gene symbol CD247
Gene ID (NCBI) 919
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD247, also known as CD3Z (T-cell surface glycoprotein CD3 zeta chain), belongs to the CD3Z/FCER1G family. CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.