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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD25/IL2RA Polyclonal antibody

CD25/IL2RA Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30449-1-AP

Synonyms

CD25, IL-2 receptor subunit alpha, IL2 RA, IL 2R subunit alpha, IL 2 receptor subunit alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD25/IL2RA Polyclonal antibody (30449-1-AP)

    WB analysis using 30449-1-AP

    MJ cells were subjected to SDS PAGE followed by western blot with 30449-1-AP (CD25/IL2RA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inMJ cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30449-1-AP targets CD25/IL2RA in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name interleukin 2 receptor, alpha
Calculated molecular weight 272 aa, 31 kDa
Observed molecular weight55-65 kDa
GenBank accession numberBC156627
Gene symbol IL2RA
Gene ID (NCBI) 3559
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+ Foxp3+ Treg cells (PMID: 22585674).

Protocols

Product Specific Protocols
WB protocol for CD25/IL2RA antibody 30449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols