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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD26 (BA5b) Mouse IgG2a Recombinant Antibody

CD26 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

BA5b

Cat no : 65574-1-MR

Synonyms

ADABP, ADCP 2, ADCP2, CD26, dipeptidyl peptidase 4, Dipeptidyl peptidase IV, DPP IV, DPP4, DPPIV, T cell activation antigen CD26, TP103

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD26 (BA5b) Mouse IgG2a Recombinant Ant (65574-1-MR)

    FC experiment of human PBMCs using 65574-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD26 Mouse IgG2a Recombinant Antibody (65574-1-MR, Clone: BA5b) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD26 (BA5b) Mouse IgG2a Recombinant Ant (65574-1-MR)

    FC experiment of human PBMCs using 65574-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD26 Mouse IgG2a Recombinant Antibody (65574-1-MR, Clone: BA5b) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD26 (BA5b) Mouse IgG2a Recombinant Ant (65574-1-MR)

    FC experiment of human PBMCs using 65574-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD26 Mouse IgG2a Recombinant Antibody (65574-1-MR, Clone: BA5b) (left) or 0.25 ug Anti-Human CD26 (65267-1-Ig, Clone: BA5b) (right) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65574-1-MR targets CD26 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen A human T cell clone 相同性解析による交差性が予測される生物種
Full Name dipeptidyl-peptidase 4
Calculated molecular weight 88 kDa
GenBank accession numberBC013329
Gene symbol DPP4
Gene ID (NCBI) 1803
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580).

Protocols

Product Specific Protocols
FC protocol for CD26 antibody 65574-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols