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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD27 (O323) Mouse IgG2a Recombinant Antibody, PBS Only

CD27 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

O323

Cat no : 65627-1-PBS

Synonyms

T cell activation antigen CD27, S152, O323, CD27L receptor, CD27 molecule

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD27 (O323) Mouse IgG2a Recombinant Ant (65627-1-MR)

    FC experiment of human PBMCs using 65627-1-MR (same clone as 65627-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD27 (O323) Mouse IgG2a Recombinant Antibody (65627-1-MR, Clone:O323) or 0.25 ug Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with APC Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65627-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD27 (O323) Mouse IgG2a Recombinant Ant (65627-1-MR)

    FC experiment of human PBMCs using 65627-1-MR (same clone as 65627-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD27 (O323) Mouse IgG2a Recombinant Antibody (65627-1-MR, Clone: O323) (red) or 0.25 ug Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65627-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65627-1-PBS targets CD27 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name CD27 molecule
Calculated molecular weight 29 kDa
GenBank accession numberBC012160
Gene symbol CD27
Gene ID (NCBI) 939
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.