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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD27 Monoclonal antibody, PBS Only

CD27 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C1G3

Cat no : 66308-1-PBS

Synonyms

CD27, CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27, T14, TNFRSF7, Tp55

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD27 Monoclonal antibody (66308-1-Ig)

    WB analysis using 66308-1-Ig (same clone as 66308-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66308-1-Ig (CD27 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig spleen tissue using CD27 Monoclonal antibody (66308-1-Ig)

    WB analysis of pig spleen using 66308-1-Ig (same clone as 66308-1-PBS)

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 66308-1-Ig (CD27 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD27 Monoclonal antibody (66308-1-Ig)

    IHC staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66308-1-Ig (CD27 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD27 Monoclonal antibody (66308-1-Ig)

    IHC staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66308-1-Ig (CD27 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD27 Monoclonal antibody (66308-1-Ig)

    IF Staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66308-1-Ig (CD27 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD27 Monoclonal antibody (66308-1-Ig)

    IF Staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66308-1-Ig (CD27 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66308-1-PBS targets CD27 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD27 fusion protein Ag24537 相同性解析による交差性が予測される生物種
Full Name CD27 molecule
Calculated molecular weight 29 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC012160
Gene symbol CD27
Gene ID (NCBI) 939
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.