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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD27 Monoclonal antibody (66308-1-Ig)

    WB analysis using 66308-1-Ig (same clone as 66308-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66308-1-Ig (CD27 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig spleen tissue using CD27 Monoclonal antibody (66308-1-Ig)

    WB analysis of pig spleen using 66308-1-Ig (same clone as 66308-1-PBS)

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 66308-1-Ig (CD27 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD27 Monoclonal antibody (66308-1-Ig)

    IHC staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66308-1-Ig (CD27 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD27 Monoclonal antibody (66308-1-Ig)

    IHC staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66308-1-Ig (CD27 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD27 Monoclonal antibody (66308-1-Ig)

    IHC staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66308-1-Ig (CD27 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD27 Monoclonal antibody (66308-1-Ig)

    IF Staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66308-1-Ig (CD27 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD27 Monoclonal antibody (66308-1-Ig)

    IF Staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66308-1-Ig (CD27 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using CD27 Monoclonal antibody (66308-1-Ig)

    FC experiment of human PBMCs using 66308-1-Ig (same clone as 66308-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.2 μg CD27 Monoclonal antibody (66308-1-Ig, Clone:1C1G3) or 0.2 μg Mouse IgG1 isotype control (66360-1-Ig, Clone: T1F8D3F10), and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO.RGAM005). Cells were not fixed. This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

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CD27 Monoclonal antibody, PBS Only

CD27 Monoclonal Antibody for WB, IHC, IF-P, FC, ELISA
Cat No. 66308-1-PBS
Clone No.1C1G3

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF-P, FC, ELISA

Synonyms

T cell activation antigen CD27, S152, CD27L receptor, CD27 molecule, CD27 antigen

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66308-1-PBS targets CD27 in WB, IHC, IF-P, FC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD27 fusion protein Ag24537 相同性解析による交差性が予測される生物種
Full Name CD27 molecule
Calculated molecular weight 29 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC012160
Gene Symbol CD27
Gene ID (NCBI) 939
ENSEMBL Gene IDENSG00000139193
RRIDAB_2881690
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26842
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.