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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PE Anti-Human PD-1/CD279 (EH12.2H7)

PD-1/CD279 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Non-human primates, Human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

EH12.2H7

Cat no : PE-65168

Synonyms

CD279, hPD 1, hPD l, PD1, PDCD1, programmed cell death 1, Protein PD 1, SLEB2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using PE Anti-Human PD-1/CD279 (EH12.2H7) (PE-65168)

    FC experiment of human PBMCs using PE-65168

    1X10^6 PHA treated (3d) human PBMCs were surface co-stained with CL750 Anti-Human CD3 and 5 ul PE Anti-Human PD-1/CD279 (PE-65168, Clone:EH12.2H7) (right) or Mouse IgG1 Isotype Control (left). 1X10^6 untreated human PBMCs were surface co-stained with CL750 Anti-Human CD3 and 5 ul PE Anti-Human PD-1/CD279 (PE-65168, Clone:EH12.2H7) (center). Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using PE Anti-Human PD-1/CD279 (EH12.2H7) (PE-65168)

    FC experiment of human PBMCs using PE-65168

    1X10^6 PHA treated (3d) human PBMCs were surface stained with 5 ul PE Anti-Human PD-1/CD279 (PE-65168, Clone:EH12.2H7) (red) or Mouse IgG1 Isotype Control (blue). 1X10^6 untreated human PBMCs were surface stained with 5 ul PE Anti-Human PD-1/CD279 (PE-65168, Clone:EH12.2H7) (black dashed). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inPHA treated human PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65168 targets PD-1/CD279 in FC applications and shows reactivity with Non-human primates, Human samples.

Tested Reactivity Non-human primates, Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name programmed cell death 1
Calculated molecular weight 288 aa, 32 kDa
GenBank accession numberBC074740
Gene symbol PD-1
Gene ID (NCBI) 5133
RRIDAB_2883944
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodThe purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).

Protocols

Product Specific Protocols
FC protocol for PE PD-1/CD279 antibody PE-65168Download protocol
Standard Protocols
Click here to view our Standard Protocols