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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD28 Recombinant antibody, PBS Only (Detector)

CD28 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240353H7

Cat no : 83383-2-PBS

Synonyms

Tp44, T-cell-specific surface glycoprotein CD28, CD28 molecule, 240353H7

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00136-5

    Sandwich ELISA standard curve of MP00136-5

    Sandwich ELISA standard curve of MP00136-5, Human CD28 Monoclonal Matched Antibody Pair - PBS only. 83383-8-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0889. 83383-2-PBS was HRP conjugated as the detection antibody. Range: 78.125-5000 pg/mL.

  • Sample test of MP00136-5

    Sample test of MP00136-5

    Serum of sixteen individual healthy human donors were measured. The human CD28 concentration of detected samples was determined to be 879.1 pg/mL with a range of 287.8 - 2,540.7 pg/mL.

  • Cytometric bead array standard curve of MP00136-4

    Cytometric bead array standard curve of MP00136-4

    Cytometric bead array standard curve of MP00136-4, CD28 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83383-3-PBS. Detection antibody: 83383-2-PBS. Standard: Eg0889. Range: 0.313-40 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83383-2-PBS targets CD28 as part of a matched antibody pair:

MP00136-4: 83383-3-PBS capture and 83383-2-PBS detection (validated in Cytometric bead array)

MP00136-5: 83383-8-PBS capture and 83383-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD28 molecule
Calculated molecular weight 220 aa, 25 kDa
GenBank accession numberBC093698
Gene symbol CD28
Gene ID (NCBI) 940
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.