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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD28 Recombinant antibody

CD28 Recombinant Antibody for IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, ELISA

Conjugate

Unconjugated

CloneNo.

240354D2

Cat no : 83383-6-RR

Synonyms

CD28, CD28 molecule, Tp44

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CD28 Recombinant antibody (83383-6-RR)

    IF Staining of Jurkat using 83383-6-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells and THP-1 cells (negative control) using CD28 antibody (83383-6-RR, Clone: 240354D2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CD28 Recombinant antibody (83383-6-RR)

    IF Staining of Jurkat using 83383-6-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells and THP-1 cells (negative control) using CD28 antibody (83383-6-RR, Clone: 240354D2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Affinity experiments of 83383-6-RR

    Affinity experiments of 83383-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83383-6-RR against Human CD28 were performed. The affinity constant is 1.99 nM.

Tested Applications

Positive IF detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83383-6-RR is CD28 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD28 molecule
Calculated molecular weight 220 aa, 25 kDa
GenBank accession numberBC093698
Gene symbol CD28
Gene ID (NCBI) 940
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD28 (T-cell-specific surface glycoprotein CD28), also known as T44 and Tp44, is a 44 kD disulfide-linked homodimeric type I glycoprotein (PMID: 2162180). It is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells (PMID: 2162180, 8386518). CD28 may affect in vivo immune responses by functioning both as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway (PMID: 2162180, 3021470). CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop (PMID: 15696168, 7964482). CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death (PMID: 1348520).

Protocols

Product Specific Protocols
IF protocol for CD28 antibody 83383-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols