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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD28 Rabbit Recombinant Antibody, PBS Only

CD28 Recombinant Antibody for Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240354D2

Cat no : 98032-1-PBS

Synonyms

Tp44, T-cell-specific surface glycoprotein CD28, CD28 molecule, 240354D2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD28 Rabbit Recombinant Antibody (98032-1-RR)

    FC experiment of human PBMCs using 98032-1-RR (same clone as 98032-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD28 Rabbit Recombinant Antibody (98032-1-RR, Clone: 240354D2) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cell were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98032-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD28 Rabbit Recombinant Antibody (98032-1-RR)

    FC experiment of human PBMCs using 98032-1-RR (same clone as 98032-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD28 Rabbit Recombinant Antibody (98032-1-RR, Clone: 240354D2) (red) or Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98032-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98032-1-RR

    Affinity and Kinetic Characterization of 98032-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98032-1-RR against Human CD28 were performed. The affinity constant is 3.23 nM.

Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 98032-1-PBS is CD28 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD28 molecule
Calculated molecular weight 220 aa, 25 kDa
GenBank accession numberBC093698
Gene symbol CD28
Gene ID (NCBI) 940
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.