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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD29 (TS2/16) Mouse IgG2a Recombinant A (65601-1-MR)

    FC experiment of human PBMCs using 65601-1-MR (same clone as 65601-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD29 (TS2/16) Mouse IgG2a RecAb (65601-1-MR, Clone: TS2/16) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with APC Anti-Human CD45. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65601-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD29 (TS2/16) Mouse IgG2a Recombinant A (65601-1-MR)

    FC experiment of human PBMCs using 65601-1-MR (same clone as 65601-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD29 (TS2/16) Mouse IgG2a RecAb (65601-1-MR, Clone: TS2/16) (red) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65601-1-PBS in a different storage buffer formulation.

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Anti-Human CD29 (TS2/16) Mouse IgG2a Recombinant Antibody, PBS Only

CD29 Uni-rAbTM Recombinant Antibody for FC
Cat No. 65601-1-PBS
Clone No.TS2/16

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

ITGB1, ITB1, Integrin beta-1, Integrin beta 1, GPIIA

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65601-1-PBS targets CD29 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Calculated molecular weight 88 kDa
GenBank accession numberBC020057
Gene Symbol Integrin beta 1
Gene ID (NCBI) 3688
ENSEMBL Gene IDENSG00000150093
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.