FITC Anti-Mouse CD3ε (500-A2)
CD3ε Monoclonal Antibody for FC
Host / Isotype
Hamster / IgG
Reactivity
Mouse
Applications
FC
Conjugate
FITC Fluorescent Dye
CloneNo.
500-A2
Cat no : FITC-65061
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
FITC-65061 targets CD3ε in FC applications and shows reactivity with Mouse samples.
| Tested Reactivity | Mouse |
| Host / Isotype | Hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Immune complexes isolated from C6VL-BS thymoma cells 相同性解析による交差性が予測される生物種 |
| Full Name | CD3 antigen, epsilon polypeptide |
| GenBank accession number | BC098236 |
| Gene symbol | CD3ε |
| Gene ID (NCBI) | 12501 |
| RRID | AB_2883751 |
| Conjugate | FITC Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 498 nm / 526 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC CD3ε antibody FITC-65061 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
