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  • Featured Product
  • KD/KO Validated

CD3 Monoclonal antibody, PBS Only (Detector)

CD3 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F3A1

Cat no : 60181-1-PBS

Synonyms

CD3E, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, CD3-epsilon, CD3 epsilon

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CD3 Monoclonal antibody (60181-1-Ig)

    WB analysis of Jurkat using 60181-1-Ig (same clone as 60181-1-PBS)

    WB result of CD3 antibody (60181-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD3 transfected Jurkat cells. This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD3 Monoclonal antibody (60181-1-Ig)

    WB analysis using 60181-1-Ig (same clone as 60181-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60181-1-Ig (CD3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD3 Monoclonal antibody (60181-1-Ig)

    IHC staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60181-1-Ig(CD3E antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD3 Monoclonal antibody (60181-1-Ig)

    IHC staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60181-1-Ig(CD3E antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using CD3 Monoclonal antibody (60181-1-Ig)

    IHC staining of human colon using 60181-1-Ig (same clone as 60181-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60181-1-Ig(CD3E antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using CD3 Monoclonal antibody (60181-1-Ig)

    IHC staining of human colon using 60181-1-Ig (same clone as 60181-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60181-1-Ig(CD3E antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD3 Monoclonal antibody (60181-1-Ig)

    IF Staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (60181-1-Ig, Clone: 3F3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), CD20 antibody (24828-1-AP, green). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD3 Monoclonal antibody (60181-1-Ig)

    IF Staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (60181-1-Ig, Clone: 3F3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), CD20 antibody (24828-1-AP, green). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD3 Monoclonal antibody (60181-1-Ig)

    IF Staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (60181-1-Ig, Clone: 3F3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), CD20 antibody (24828-1-AP, green). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD3 Monoclonal antibody (60181-1-Ig)

    IF Staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60181-1-Ig (CD3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CD3 Monoclonal antibody (60181-1-Ig)

    IF Staining of Jurkat using 60181-1-Ig (same clone as 60181-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD3 antibody (60181-1-Ig, Clone: 3F3A1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50222-1

    Cytometric bead array standard curve of MP50222-1

    Cytometric bead array standard curve of MP50222-1, CD3E Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60181-2-PBS. Detection antibody: 60181-1-PBS. Standard:Ag11797. Range: 0.098-50 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60181-1-PBS targets CD3 as part of a matched antibody pair:

MP50222-1: 60181-2-PBS capture and 60181-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD3 fusion protein Ag11797 相同性解析による交差性が予測される生物種
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Calculated molecular weight 207 aa, 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC049847
Gene symbol CD3
Gene ID (NCBI) 916
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a monoclonal antibody directed against the epsilon chain of human CD3 molecule.