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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD3 (OKT3) Rabbit Recombinant Antibody, PBS only

CD3 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

OKT3

Cat no : 65504-1-PBS

Synonyms

CD3E, T3E, TCRE

Validation Data Gallery

Filter:
  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD3 (OKT3) (65504-1-PBS)

    FC experiment of human PBMCs using 65504-1-PBS

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD3 Rabbit Recombinant Antibody (65504-1-PBS, Clone:OKT3) or Rabbit IgG control PolyAb, and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD45. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD3 (OKT3) Rabbit Recombinant Antibody (65504-1-PBS)

    FC experiment of human PBMCs using 65504-1-PBS

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD3 Rabbit Recombinant Antibody (65504-1-PBS, Clone:OKT3) or Rabbit IgG control PolyAb, and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD3 (OKT3) Rabbit Recombinant Antibody (65504-1-PBS)

    FC experiment of human PBMCs using 65504-1-PBS

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD3 Rabbit Recombinant Antibody (65504-1-PBS, Clone:OKT3) (left) and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 or 0.2ug Anti-Human CD3 Mouse Monoclonal Antibdody (Clone:OKT3) (center and right) and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD45. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65504-1-PBS targets CD3 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Human T cells 相同性解析による交差性が予測される生物種
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Calculated molecular weight 207 aa, 23 kDa
GenBank accession numberBC049847
Gene symbol CD3
Gene ID (NCBI) 916
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocols

Product Specific Protocols
FC protocol for CD3 antibody 65504-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols