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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD3 (UCHT1) Mouse IgG1 Recombinant Anti (65536-1-MR)

    FC experiment of human PBMCs using 65536-1-MR (same clone as 65536-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD3 (65536-1-MR, Clone:UCHT1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.25 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (blue). Cells were not fixed. This data was developed using the same antibody clone with 65536-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD3 (UCHT1) Mouse IgG1 Recombinant Anti (65536-1-PBS)

    FC experiment of human PBMCs using 65536-1-PBS

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD3 (65536-1-PBS, Clone: UCHT1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.25 ug Isotype Control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (blue). Cells were not fixed.

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Anti-Human CD3 (UCHT1) Mouse IgG1 Recombinant Antibody, PBS Only

CD3 Recombinant Antibody for FC
Cat No. 65536-1-PBS
Clone No.UCHT1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC

Synonyms

CD3E, CD3 epsilon, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, T3E

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65536-1-PBS targets CD3 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Recombinant
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Calculated molecular weight 207 aa, 23 kDa
GenBank accession numberBC049847
Gene Symbol CD3
Gene ID (NCBI) 916
ENSEMBL Gene IDENSG00000198851
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocols

Product Specific Protocols
FC protocol for CD3 antibody 65536-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols