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  • Featured Product
  • KD/KO Validated

CD3 Recombinant antibody

CD3 Recombinant Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

4I19

Cat no : 81324-1-RR

Synonyms

CD3E, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, CD3-epsilon, CD3 epsilon

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CD3 Recombinant antibody (81324-1-RR)

    WB analysis of Jurkat using 81324-1-RR

    WB result of CD3 antibody (81324-1-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD3 transfected Jurkat cells.

  • Western Blot (WB) analysis of various lysates using CD3 Recombinant antibody (81324-1-RR)

    WB analysis using 81324-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81324-1-RR (CD3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using CD3 Recombinant antibody (81324-1-RR)

    IHC staining of human colon using 81324-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 81324-1-RR (CD3 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using CD3 Recombinant antibody (81324-1-RR)

    IHC staining of human colon using 81324-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 81324-1-RR (CD3 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using CD3 Recombinant antibody (81324-1-RR)

    IHC staining of human colon using 81324-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 81324-1-RR (CD3 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CD3 Recombinant antibody (81324-1-RR)

    IF Staining of mouse spleen using 81324-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD3 antibody (81324-1-RR, Clone: 4I19 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 81324-1-RR

    Affinity and Kinetic Characterization of 81324-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81324-1-RR against Human CD3 were performed. The affinity constant is 1.13 nM.

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Tested Applications

Positive WB detected inJurkat cells, MOLT-4 cells, pig thymus tissue, rat thymus tissue, mouse thymus tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81324-1-RR targets CD3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CD3 fusion protein Ag11797 相同性解析による交差性が予測される生物種
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Calculated molecular weight 207 aa, 23 kDa
Observed molecular weight23-25 kDa
GenBank accession numberBC049847
Gene symbol CD3
Gene ID (NCBI) 916
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a monoclonal antibody directed against the epsilon chain of human CD3 molecule.

Protocols

Product Specific Protocols
WB protocol for CD3 antibody 81324-1-RRDownload protocol
IHC protocol for CD3 antibody 81324-1-RRDownload protocol
IF protocol for CD3 antibody 81324-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols