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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD3 Recombinant antibody, PBS Only

CD3 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242012G2

Cat no : 84505-4-PBS

Synonyms

CD3E, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, CD3-epsilon, CD3 epsilon

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD3 Recombinant antibody (84505-4-RR)

    WB analysis using 84505-4-RR (same clone as 84505-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84505-4-RR (CD3E antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84505-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CD3 Recombinant antibody (84505-4-RR)

    IF Staining of Jurkat using 84505-4-RR (same clone as 84505-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD3 antibody (84505-4-RR, Clone: 242012G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84505-4-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84505-4-PBS targets CD3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein 相同性解析による交差性が予測される生物種
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Calculated molecular weight 207 aa, 23 kDa
Observed molecular weight23 kDa
GenBank accession numberBC049847
Gene symbol CD3
Gene ID (NCBI) 916
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This antibody is directed against the epsilon chain of human CD3 molecule.