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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD3 Delta Polyclonal antibody

CD3 Delta Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 32144-1-AP

Synonyms

CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain, T3D, T cell receptor T3 delta chain

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD3 Delta Polyclonal antibody (32144-1-AP)

    WB analysis using 32144-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32144-1-AP (CD3 Delta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD3 Delta Polyclonal antibody (32144-1-AP)

    IHC staining of human tonsillitis using 32144-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 32144-1-AP (CD3 Delta antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells, MOLT-4 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32144-1-AP targets CD3 Delta in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD3d molecule, delta (CD3-TCR complex)
Calculated molecular weight 171 aa, 19 kDa
Observed molecular weight21 kDa
GenBank accession numberBC070321
Gene symbol CD3 Delta
Gene ID (NCBI) 915
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.

Protocols

Product Specific Protocols
WB protocol for CD3 Delta antibody 32144-1-APDownload protocol
IHC protocol for CD3 Delta antibody 32144-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols