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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD30/TNFRSF8 Rabbit Recombinant Antibody

CD30/TNFRSF8 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241330A12

Cat no : 98124-1-RR

Synonyms

CD30, 241330A12, Ki 1 antigen, Ki 1, D1S166E

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD30 Rabbit Recombinant Antibody (98124-1-RR)

    FC experiment of human PBMCs using 98124-1-RR

    1x10^6 PHA-treated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Rabbit Recombinant Antibody (98124-1-RR, Clone: 241330A12) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), 0.25 ug or Isotype Control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (blue). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Rabbit Recombinant Antibody (98124-1-RR, Clone: 241330A12) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98124-1-RR

    Affinity and Kinetic Characterization of 98124-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98124-1-RR against Human CD30 were performed. The affinity constant is 0.528 nM.

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Tested Applications

Positive FC detected inPHA treated human PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98124-1-RR targets CD30/TNFRSF8 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 8
Calculated molecular weight 64 kDa
GenBank accession numberNM_001243
Gene symbol CD30
Gene ID (NCBI) 943
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

CD30, also known as TNFRSF8 or Ki-1, is a transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. It is composed of an extracellular domain with six cysteine-rich repeats, a transmembrane segment, and an intracellular domain (PMID: 1310894). CD30 is expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin's lymphoma and anaplastic large cell lymphoma (PMID: 9826579; 28885612). The CD30 ligand (CD30L or CD153) is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily (PMID: 8701986). Signal transduction of CD30/CD30L utilizes signal transducers, TNFR-associated factors (TRAF1, 2, 3 and 5) (PMID: 9826579).

Protocols

Product Specific Protocols
FC protocol for CD30/TNFRSF8 antibody 98124-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols